P5: 5' AAT GAT ACG GCG ACC ACC GA 3' P7: 5' CAA GCA GAA GAC GGC ATA CGA GAT 3' When checking your libraries by cloning and stand
![Dual-index PCR primers designed for paired-end Illumina sequencing of... | Download Scientific Diagram Dual-index PCR primers designed for paired-end Illumina sequencing of... | Download Scientific Diagram](https://www.researchgate.net/profile/Iwona-Bukowska/publication/289734765/figure/fig5/AS:670527530213380@1536877629110/Dual-index-PCR-primers-designed-for-paired-end-Illumina-sequencing-of-5-UTR-region-The.jpg)
Dual-index PCR primers designed for paired-end Illumina sequencing of... | Download Scientific Diagram
![phylogenetics - Why do NEBNext indexing primers have sequence between the p5 oligo and index? - Bioinformatics Stack Exchange phylogenetics - Why do NEBNext indexing primers have sequence between the p5 oligo and index? - Bioinformatics Stack Exchange](https://i.stack.imgur.com/EhUwu.png)
phylogenetics - Why do NEBNext indexing primers have sequence between the p5 oligo and index? - Bioinformatics Stack Exchange
![Oligonucleotide design and products of protocol. P5 and P7 are names... | Download Scientific Diagram Oligonucleotide design and products of protocol. P5 and P7 are names... | Download Scientific Diagram](https://www.researchgate.net/publication/248383093/figure/fig2/AS:213955495632902@1428022373258/Oligonucleotide-design-and-products-of-protocol-P5-and-P7-are-names-given-by-Illumina-to.png)
Oligonucleotide design and products of protocol. P5 and P7 are names... | Download Scientific Diagram
![Illumina Sequencing (for Dummies) -An overview on how our samples are sequenced. – kscbioinformatics Illumina Sequencing (for Dummies) -An overview on how our samples are sequenced. – kscbioinformatics](https://kscbioinformatics.files.wordpress.com/2017/02/r1andr2.png)
Illumina Sequencing (for Dummies) -An overview on how our samples are sequenced. – kscbioinformatics
![Adapterama I: universal stubs and primers for 384 unique dual-indexed or 147,456 combinatorially-indexed Illumina libraries (iTru & iNext) [PeerJ] Adapterama I: universal stubs and primers for 384 unique dual-indexed or 147,456 combinatorially-indexed Illumina libraries (iTru & iNext) [PeerJ]](https://dfzljdn9uc3pi.cloudfront.net/2019/7755/1/fig-4-2x.jpg)
Adapterama I: universal stubs and primers for 384 unique dual-indexed or 147,456 combinatorially-indexed Illumina libraries (iTru & iNext) [PeerJ]
![phylogenetics - Why do NEBNext indexing primers have sequence between the p5 oligo and index? - Bioinformatics Stack Exchange phylogenetics - Why do NEBNext indexing primers have sequence between the p5 oligo and index? - Bioinformatics Stack Exchange](https://i.stack.imgur.com/LHZV0.jpg)
phylogenetics - Why do NEBNext indexing primers have sequence between the p5 oligo and index? - Bioinformatics Stack Exchange
![Primer construct and amplification products. The primers are composed... | Download Scientific Diagram Primer construct and amplification products. The primers are composed... | Download Scientific Diagram](https://www.researchgate.net/publication/283456688/figure/fig2/AS:613934851686442@1523384883596/Primer-construct-and-amplification-products-The-primers-are-composed-of-sequences.png)
Primer construct and amplification products. The primers are composed... | Download Scientific Diagram
Construction principle of the two (P5 and P7) sequencing adapters (top... | Download Scientific Diagram
![Illumina Sequencing (for Dummies) -An overview on how our samples are sequenced. – kscbioinformatics Illumina Sequencing (for Dummies) -An overview on how our samples are sequenced. – kscbioinformatics](https://kscbioinformatics.files.wordpress.com/2017/02/fragmentsonflowcell.png?w=319&h=343)
Illumina Sequencing (for Dummies) -An overview on how our samples are sequenced. – kscbioinformatics
![Comparing data from two Illumina chemistries (16S amplicon sequencing) · Issue #509 · benjjneb/dada2 · GitHub Comparing data from two Illumina chemistries (16S amplicon sequencing) · Issue #509 · benjjneb/dada2 · GitHub](https://user-images.githubusercontent.com/40797916/43652529-1d5d1068-9763-11e8-80b7-e8bd933ab390.png)
Comparing data from two Illumina chemistries (16S amplicon sequencing) · Issue #509 · benjjneb/dada2 · GitHub
![phylogenetics - Why do NEBNext indexing primers have sequence between the p5 oligo and index? - Bioinformatics Stack Exchange phylogenetics - Why do NEBNext indexing primers have sequence between the p5 oligo and index? - Bioinformatics Stack Exchange](https://i.stack.imgur.com/7Gc73.png)