![SOLVED: QNo.3. Calculate Tm of the following primers: Gene: Sequence Melting temperature Forward primer: TCGAGTCGCGTCCACC Reverse primer: GGGAGCATCGTCGCCC Amplified product length = b. Screen shot of in-silico PCR = Cytogenetic location = SOLVED: QNo.3. Calculate Tm of the following primers: Gene: Sequence Melting temperature Forward primer: TCGAGTCGCGTCCACC Reverse primer: GGGAGCATCGTCGCCC Amplified product length = b. Screen shot of in-silico PCR = Cytogenetic location =](https://cdn.numerade.com/ask_images/479db41e130a4d759330f82f59f9f3f4.jpg)
SOLVED: QNo.3. Calculate Tm of the following primers: Gene: Sequence Melting temperature Forward primer: TCGAGTCGCGTCCACC Reverse primer: GGGAGCATCGTCGCCC Amplified product length = b. Screen shot of in-silico PCR = Cytogenetic location =
![Primer design strategy for denaturation bubble-mediated strand exchange amplification - ScienceDirect Primer design strategy for denaturation bubble-mediated strand exchange amplification - ScienceDirect](https://ars.els-cdn.com/content/image/1-s2.0-S0003269719312813-fx1.jpg)
Primer design strategy for denaturation bubble-mediated strand exchange amplification - ScienceDirect
![Melting Temperature Mapping Method: A Novel Method for Rapid Identification of Unknown Pathogenic Microorganisms within Three Hours of Sample Collection | Scientific Reports Melting Temperature Mapping Method: A Novel Method for Rapid Identification of Unknown Pathogenic Microorganisms within Three Hours of Sample Collection | Scientific Reports](https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fsrep12543/MediaObjects/41598_2015_Article_BFsrep12543_Fig1_HTML.jpg)
Melting Temperature Mapping Method: A Novel Method for Rapid Identification of Unknown Pathogenic Microorganisms within Three Hours of Sample Collection | Scientific Reports
![Sequences and Tm of primers, and numbers and sizes of the amplified DNA... | Download Scientific Diagram Sequences and Tm of primers, and numbers and sizes of the amplified DNA... | Download Scientific Diagram](https://www.researchgate.net/publication/336250901/figure/tbl2/AS:810103502557186@1570155134237/Sequences-and-Tm-of-primers-and-numbers-and-sizes-of-the-amplified-DNA-fragments-of-L.png)